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Nahla Mohamed Hassan Mohamed

Nahla Mohamed Hassan Mohamed

University of Khartoum in Fulfillment of the Requirements for the Degree of Ph.D. in Animal Production (Animal Nutrition

Title: Effect of Vitamin E (alfa-tocopherol) Supplementation on Hemato-biochemical Profile of Race Stallions Horses

Biography

Biography: Nahla Mohamed Hassan Mohamed

Abstract

This study was conducted with the objective of determining the effect of vitamin E (α-tocopherol) supplementation on hemato-biochemical Parameters of stallion race horses in Khartoum State. The field study was carried out using 21 animals of gelding stallions race horses, thoroughbred, with average body weight 300 ± 60 kg and average age was 7 years. The horses were housed in three stables and all animals were fed green alfalfa and concentrates twice a day. The concentrate was supplemented with three levels of Vitamin E (0, 2000 IU, 3000 IU kg /feed) and 7animals were a assigned for each treatment. The experiment was carried out in two periods, the first during the period from November to December 2016 and the second from March to April 2017. Blood samples were collected from the jugular vein at the end experiments in both winter and summer season. Blood samples and serum were used to determine hemato-biochemical parameters. Statistical Analysis of data used SPSS for Windows version 20.0. Factorial Randomized Design with 2×3 (2 seasons and 3 levels of vitamin E) was used to conduct the experiments. The results indicated that with respect to the hematology parameters: HB, MCH, MCHC MCV, PCV, and TRBC increased significantly (P≤ 0.01) with increasing the level of vit. E in both winter and summer seasons. Increasing the level of vit. E resulted in positive decreased and significant differences (P≤ 0.01) in TWBC in summer season, and neutrophil and Basophil in winter season. The results of biochemical parameters: triglyceride, cholesterol (LDL, VLDL), albumin, globulin and uric acid decreased significantly (P≤ 0.01), while glucose and HDL increased in both seasons. Moreover, a significant (P≤ 0.01) decreased were detected in the liver enzymes AST, ALT, ALP and kidney function test This study was conducted with the objective of determining the effect of vitamin E (α-tocopherol) supplementation on hemato-biochemical Parameters of stallion race horses in Khartoum State. The field study was carried out using 21 animals of gelding stallions race horses, thoroughbred, with average body weight 300 ± 60 kg and average age was 7 years. The horses were housed in three stables and all animals were fed green alfalfa and concentrates twice a day. The concentrate was supplemented with three levels of Vitamin E (0, 2000 IU, 3000 IU kg /feed) and 7animals were a assigned for each treatment. The experiment was carried out in two periods, the first during the period from November to December 2016 and the second from March to April 2017. Blood samples were collected from the jugular vein at the end experiments in both winter and summer season. Blood samples and serum were used to determine hemato-biochemical parameters. Statistical Analysis of data used SPSS for Windows version 20.0. Factorial Randomized Design with 2×3 (2 seasons and 3 levels of vitamin E) was used to conduct the experiments. The results indicated that with respect to the hematology parameters: HB, MCH, MCHC MCV, PCV, and TRBC increased significantly (P≤ 0.01) with increasing the level of vit. E in both winter and summer seasons. Increasing the level of vit. E resulted in positive decreased and significant differences (P≤ 0.01) in TWBC in summer season, and neutrophil and Basophil in winter season. The results of biochemical parameters: triglyceride, cholesterol (LDL, VLDL), albumin, globulin and uric acid decreased significantly (P≤ 0.01), while glucose and HDL increased in both seasons. Moreover, a significant (P≤ 0.01) decreased were detected in the liver enzymes AST, ALT, ALP and kidney function test.